Service
- Custom DNA oligo synthesis up to 150 bases.
- Your choice of plate or tube for optimal flexibility.
- Standard DNA oligos are shipped within 2-3 working days.
- Purified & modified oligos are shipped within 4 business days.
Turnaround Time: Our Oligo-Rapid Synthesis Service provides different nmol scales 25, 50, 100, 200 and 1000 nmol for standard, desalted oligos. Turnaround times depend on sequence, purification and quantity requested, however standard oligos are shipped within two to three working days.
Fluorescent Dye and Standard Modification
| Modification | Labeling Position | |
|---|---|---|
| 5’ | 3’ | |
| FAM | ✔ | ✔ |
| TET | ✔ | - |
| HEX | ✔ | - |
| TAMRA | ✔ | ✔ |
| Cy3 | ✔ | - |
| Fluroscenin | ✔ | - |
| BHQ | - | ✔ |
| Standard Modification | |||
|---|---|---|---|
| Modification | Labeling Position | ||
| 5’ | 3’ | Internal | |
| Biotin | ✔ | ✔ | - |
| Inosine | ✔ | - | ✔ |
| Aminolink | ✔ | ✔ | - |
| Thiol | ✔ | ✔ | - |
| Phosphorylation | ✔ | ✔ | - |
Oligo purification
| Purification Type | Final Yield (based on oligo length) | |||
|---|---|---|---|---|
| 5-60 bases | 11-60 bases | 60-100 bases | 100-150 bases | |
| Desalted(>55% Pure) | ¯ | 10 OD | ¯ | ¯ |
| High Affinity Purification (>75% Pure) | ¯ | 5 OD | ¯ | ¯ |
| PAGE (>85% Pure)Long oligo | ¯ | ¯ | 5 OD | 5 OD |
| HPLC (>85% Pure) Long oligo | ¯ | ¯ | 5 OD | 2 OD |
- Salts removed.
- Failure sequences NOT removed.
- Purity: >55%.
- Length: 11-60 bases.
- Application: PCR, Sequencing.
- Oligos significantly more pure than the Desalted method.
- Salts removed.
- Some failure sequences removed.
- Purity: >75%.
- Length: 11-60 bases.
- Application: PCR, Sequencing.
- Polyacrylamide gel electrophoresis (PAGE) based.
- Recommended for longer oligos (>60 bases).
- Salts removed.
- Majority of failure sequences removed.
- Purity: >85%*.
- Length: 11-150 bases.
- Application: Cloning, Gel-Shift Assay, Mutagenesis.
- High Performance Liquid Chromatography (HPLC) based.
- Recommended for shorter oligos (5-59 bases).
- Salts removed.
- Majority of failure sequences removed.
- Purity: >85%*.
- Oligo length: 5-150 bases.
- Application: Cloning, Gel Shift Assay, Mutagenesis.
DNA Synthesis
- Unmodified DNA Synthesis
- Modified DNA Synthesis
- Long Oligonucleotide Synthesis
- DNA qPCR Probes
- Others According to Your Requirements
Purifications
- Unmodified DNA Synthesis
- Desalting
- High Affinity Purification
- PAGE
- HPLC
Features and Benefits
- Unmodified DNA Synthesis
- Highest Quality
- Cost Effective
- Fast
- Excellent Technical Support
- Comprehensive Service Portfolio
Get Quotations and Ordering
To request a quotation or for placing orders, please download our order form and email it to our oligo synthesis department at oligo@sahagene.com .Alternatively, you can email us your sequences directly (including purification type, yield, modifications etc.). Our department will get back to you within 24 hours (on a business day).